Semi-Pure Maintenance Diet (AIN-93M)

This diet is based on a formulation developed by the American Society of Nutritional Sciences: ASNS (formerly American Institute of Nutrition). The Diet AIN-93M was a modification of a prior formulation AIN-76A.  This well studied diet was updated to separate maintenance from growing animals and improve the long term health of rodents fed semi-pure diets.

  • The formulation for AIN-93G and AIN-93M was first presented in Journal of Nutrition., 123[11]:1939-51, November 1993,
    An Interview with Dr. Philip G. Reeves for in-cites
  • AIN93M is the maintenance form of this standard pure or semi-pure diet formulation.
  • We have made some modifications of the formulation to suit locally available raw materials.  Our experience in short and long term feeding trials confirms that these modifications have little or no impact on animal performance.
  • Cystine in the original formulation has been replaced by methionine.  Although casein has a low cystine content, there is good evidence of interconversion of methionine and cystine.
  • Soybean oil has been replaced by Canola oil.  While this changes the fatty acid profile of the diet all known requirements have been met.  The biggest difference between soybean and canola oils is in the linoleic and linolenic acid content. Although canola oil has a lower linoleic acid content (a known essential fatty acid) it could be argued that the n6 / n3 fatty acid ratio of canola oil is superior to soybean oil.  The erucic acid content of canola oils available in Western Australia is extremely low.
  • Some minor modifications have been made in the salt form of the trace minerals.
  • If you are considering irradiating this diet at 25 KGy we suggest that you consider SF08020

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Keywords: pure diet, semi-pure diet, control diet, growth, AIN93M, AIN, AIN93